For the detection of antibodies to Treponema Pallidum in human (syphilis test kit ELISA)

Model Number: Anti-TP ELISA01 Brand Name: Egens Key Specifications/Special Features: The Anti-TP ELISA is a qualitative enzyme immunoassay for the in vitro detection of Treponema Pallidum infection,based on the detection of antibodies to Treponema Pallidum in human serum or plasma. It is intended forscreening of blood donors and diagnosing patients related to infection with Treponema Pallidum.Assay procedure:1. Prepare Reagents: Dilute 1 volume of Concentrated Washing Buffer (20×)with 19 volumes of distilled water,mix well.2. Add Samples: Open the foil pouch and remove the Microplate. Set up 1 well as Blank, 2 wells as negativecontrol, 2 wells as positive control. After dispensing 50L of Sample Diluent , dispense 50L of sample ornegative control or positive control to the respective wells. Gently vibrating the plate. 3. Incubate: Cover the Microplate with plate cover and incubate the Coated Microplate in a thermostat-controlledwater-bath or microplate incubator at 37℃ for 60 minutes.
4. Wash the Plate: Remove the plate cover. Aspirate the contents of all wells. Fill the wells with the diluted
washing buffer ( 10~20 seconds to soak) then aspirate again. Repeat the procedure for 5 times. Make sure
that the rest volume is minimal, by tapping plate onto absorbent paper.
5. Add Conjugate: Add 100L of conjugate to each well (except the blank well).
6. Incubate: Cover the Microplate and incubate the plate at 37℃ for 30 minutes.
7. Wash the Plate: Repeat the wash procedure as in step 4.
8. Add Substrate: Add 50L of Substrate Solution A and 50L of Substrate Solution B to each well, mix well.
Cover and incubate at 37℃ for 10 minutes.
9. Stop reaction: Add 50L Stop Solution to each well, mix well.
10. Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should
be selected from 620nm to 690nm.